Goat anti Dog IgM (Fc specific), conjugated with Biotin, Secondary Antibodies
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Catalog numberGAD-IgM(Fc)-Bio
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Price:
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Size1ml
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Host SourceGoat
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Clone NamePolyclonal
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Product CategoryVeterinary
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Species ReactivityCanine
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ApplicationELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
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ImmunogenPurified normal IgM isolated from pooled dog serum. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
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Purification MethodNA
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Shipping ConditionsNA
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Storage conditionsGoat anti Dog IgM (Fc specific), conjugated with Biotin, Secondary Antibodies philized conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days
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Datasheet linkNA
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Canine or Canis Lupus is mostly Beagle used for drug research. Exalpha produces ELISA test kits and polyclonal antibodies. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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ConjugationBiotinylated
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Latin nameCapra aegagrus hircus
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Gene target
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Short nameGoat anti IgM (Fc specific), conjugated with , Secondary Antibodies
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Techniqueanti, Goat, IgM, FC, antibody to, antibody Conjugates, goats, IgMs
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeNA, IgM
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LabelBiotin
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SpeciesDog, Dogs, Goats
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Alternative namecaprine antibody to canine Immunoglobulin M (fragment c specific), coupled including biotinilated, secondary detector Antibody Antibodies
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Alternative techniqueantibodies, caprine, immunoglobulin-m
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data