SEMA5A Polyclonal Antibody, FITC Conjugated

Note

For research use only.
Properties

If you buy Antibodies supplied by EpiGentek they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This EpiGentek Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. EpiGentek FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation

anticorps

Gene target

SEMA5A Conjugated
Gene symbol

SEMA5A-AS1, SEMA5A
Short name

SEMA5A Polyclonal Antibody, FITC Conjugated
Technique

Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Label

FITC
Alternative name

sema domain, seven thrombospondin repeats (classification 1 and classification 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A polyclonal (antibody to-), fluorecein coupled
Alternative technique

polyclonals, antibodies, fluorescine
Alternative to gene target

sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A, SEMAF and semF, SEMA5A and IDBG-12173 and ENSG00000112902 and 9037, syndecan binding, Plasma membranes, Sema5a and IDBG-133049 and ENSMUSG00000022231 and 20356, SEMA5A and IDBG-631754 and ENSBTAG00000005021 and 506636

Identity

HGNC:10736
Gene

SEMA5A
Long gene name

semaphorin 5A
Synonyms gene
- SEMAF
Synonyms gene name
- sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Synonyms
- semF
GenBank acession
- U52840
Locus

5p15.31
Discovery year

1999-06-25
Entrez gene record

9037
Pubmed identfication
- 8817451
- 9464278
Classification
- MicroRNA protein coding host genes
- Semaphorins
VEGA ID

OTTHUMG00000090501

Name

Cytophotometry
Concept

Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data