SEMA5A Polyclonal Antibody, Biotin Conjugated

Note

For research use only.
Properties

If you buy Antibodies supplied by EpiGentek they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation

anticorps

Gene target

SEMA5A Conjugated
Gene symbol

SEMA5A-AS1, SEMA5A
Short name

SEMA5A Polyclonal Antibody, Conjugated
Technique

Polyclonal, Antibody, antibodies against human proteins, antibodies for, antibody Conjugates, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Label

Biotin
Alternative name

sema domain, seven thrombospondin repeats (classification 1 and classification 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A polyclonal (antibody to-), biotinilated coupled
Alternative technique

polyclonals, antibodies
Alternative to gene target

sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A, SEMAF and semF, SEMA5A and IDBG-12173 and ENSG00000112902 and 9037, syndecan binding, Plasma membranes, Sema5a and IDBG-133049 and ENSMUSG00000022231 and 20356, SEMA5A and IDBG-631754 and ENSBTAG00000005021 and 506636

Identity

HGNC:10736
Gene

SEMA5A
Long gene name

semaphorin 5A
Synonyms gene
- SEMAF
Synonyms gene name
- sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Synonyms
- semF
GenBank acession
- U52840
Locus

5p15.31
Discovery year

1999-06-25
Entrez gene record

9037
Pubmed identfication
- 8817451
- 9464278
Classification
- MicroRNA protein coding host genes
- Semaphorins
VEGA ID

OTTHUMG00000090501

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data