EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
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Catalog number
P-2002-3
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Price
Please ask
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Size
96 Reactions
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Note
For research use only.
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Properties
Precipitation of Chromatins is a pull down technique of native DNA sequences by specific binding proteins on which on the other side of the protein the antibody can bind. Lots of antibodies are not suited for Chip analysis pulldown because they bind on the binding site of the DNA. The EpiGentek antibody is proven to not bind on the DNA binding site and is suitable for ChiP precipitation supplied in 1 vials.
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Test
Chromatin isolation for histone extracts like H2A, H2B, H3, and H4 extraction for SDS-PAGE electrophoresis. Histones act as spools around which DNA winds, and also play a role in gene regulation. The core histones include H2A, H2B, H3, and H4. Histones undergo post-translational modifications, which alter their interaction with DNA and nuclear proteins. The H3 and H4 histones have long tails protruding from the nucleosome, which can be covalently modified at several places (i.e., methylation, acetylation, phosphorylation, etc.). Histone modifications act in diverse biological processes such as gene regulation, DNA repair and chromosome condensation (mitosis). Combinations of modifications are thought to constitute a "histone code" and an understanding of this code may help to answer questions about human development and disease.
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Gene target
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Short name
EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
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Technique
Immunoprecipitation, chromatin, Epiquik, ChiP, Chromatine immuno Precipitationg antibody, epiquik
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Alternative name
EpiQuik Chromatin Immunoprecipitation (ChIP) reagent
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Alternative technique
epigenetic, epigenetics, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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