Phosphotyrosine Monoclonal Antibody [PY20], FITC Conjugated

• Catalog number
  A-7432-100
• Price
  Please ask
• Size
  0,1 mg

• Target antigen
  Phosphotyrosine
• Clone
  PY20
• Conjugation additive
  FITC Conjugated
• Product type
  Antibody
• Clonality
  monoclonal antibody
• Long name
  Phosphotyrosine Monoclonal Antibodies [PY20], FITC Conjugated
• Description
  Phosphotyrosine. Monoclonal antibodies ensure a highly specific binding to a certain epitope of the antigen and are much more LOT-to-LOT consistent than polyclonal antibodies.
• Storage conditions
  For short term storage the Phosphotyrosine Monoclonal Antibody [PY20], FITC Conjugated can be kept refrigerated at +4 degrees Celsius. For long term storage keep frozen at -20 degrees Celsius. Be sure to refer to the technical sheet for any additional specifications and requirements for proper storing of the antibodies.
• Tips
  Phosphotyrosine MAb [PY20], FITC Conjugated should not be exposed to cycles of freezing and thawing as they may denaturate the peptide chains/bonds in the antibodies and reduce/change the activity of the antibody. Prior to use briefly centrifuge the vial to collect its content on the bottom.
• Properties
  If you buy Antibodies supplied by Epigen they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Epigen Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Epigen FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  Phosphotyrosine   Monoclonal   [PY20]   Conjugated  
• Short name
  Anti-Phosphotyrosine
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, Monoclonals or monoclonal antibodies
• Label
  FITC
• Alternative name
  Phosphotyrosine MAb [PY20], FITC Conjugated
• Alternative technique
  antibodies, fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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