Anti-CD2 Antibody [RPA-2.10], FITC-25Tests

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
Properties

If you buy Antibodies supplied by EnQuireBio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This EnQuireBio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. EnQuireBio FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody
French translation

anticorps

Gene target

CD2 [RPA-2 10] 25Tests
Gene symbol

RPA2, CD2, POLR1B, MIR7-2, MIR329-2, MIR512-2, MIR521-2, MIR1289-2, MIR509-2, KRTAP13-2
Short name

Anti-CD2 Antibody [RPA-2 10], FITC-25Tests
Technique

Antibody, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, Fluorescein
Label

FITC
Alternative name

Antibody toCD2 (antibody to-) [RPA-2.10], fluorecein-25Tests
Alternative technique

antibodies, fluorescine
Alternative to gene target

CD2 molecule, LFA-2 and SRBC and T11, CD2 and IDBG-101417 and ENSG00000116824 and 914, protein homodimerization activity, Extracellular, Cd2 and IDBG-179684 and ENSMUSG00000027863 and 12481, CD2 and IDBG-634458 and ENSBTAG00000017256 and 497022

Identity

HGNC:10290
Gene

RPA2
Long gene name

replication protein A2
Synonyms gene name
- replication protein A2 (32kD)
- replication protein A2, 32kDa
GenBank acession
- BC021257
Locus

1p35.3
Discovery year

1993-12-14
Entrez gene record

6118
Pubmed identfication
- 8454588
RefSeq identity
- NM_002946
Classification
- Nucleotide excision repair
VEGA ID

OTTHUMG00000003915

Identity

HGNC:1639
Gene

CD2
Long gene name

CD2 molecule
Synonyms gene
- SRBC
Synonyms gene name
- CD2 antigen (p50), sheep red blood cell receptor
GenBank acession
- BC033583
Locus

1p13.1
Discovery year

1986-01-01
Entrez gene record

914
Pubmed identfication
- 2437578
RefSeq identity
- NM_001767
Classification
- CD molecules
- Ig-like cell adhesion molecule family
- C2-set domain containing
- V-set domain containing
VEGA ID

OTTHUMG00000022750

Name

Fluorescent Antibody Technique
Concept

Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data