Mouse SCCA2(Squamous Cell Carcinoma Antigen 2) ELISA Kit
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Catalog number
ELK2847
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Price
Please ask
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Size
96T
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Alternative Names
SERPINB4; PI11; SPB4; Leupin; Serpin Peptidase Inhibitor,Clade B,Member 4; Peptidase Inhibitor 11
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Assay Type
Sandwich
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Sensitivity
0.056ng/mL
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Standard
10ng/mL
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Detection range
0.156-10ng/mL
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Assay length
3h
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Research Area
Tumor immunity;Gastroenterology;
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Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Squamous Cell Carcinoma Antigen 2 (SCCA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Squamous Cell Carcinoma Antigen 2 (SCCA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Squamous Cell Carcinoma Antigen 2 (SCCA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Squamous Cell Carcinoma Antigen 2 (SCCA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Description
Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency.
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
SERPINB4
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Short name
Mouse SCCA2(Squamous Carcinoma Antigen 2) ELISA Kit
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Technique
ELISA kit, antigen, ELISA, Mouse, antigenes, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
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Host
mouse, antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Mouse, Mouses
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Alternative name
Mouse SCCA2(Squamous cellular Carcinoma protein 2) Enzyme-linked immunosorbent assay reagent
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Alternative technique
antigenes, murine, elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
carcinoma, cell
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Gene info
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Identity
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Gene
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Long gene name
serpin family B member 4
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Synonyms gene
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Synonyms gene name
- squamous cell carcinoma antigen 2
- serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 4
- serpin peptidase inhibitor, clade B (ovalbumin), member 4
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Synonyms
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GenBank acession
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Locus
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Discovery year
1995-09-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serpin peptidase inhibitors
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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