Mouse SCCA2(Squamous Cell Carcinoma Antigen 2) EIA Kit
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Catalog number
E-EL-M1090-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Mouse
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Target Synonym
NA
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SCCA2. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SCCA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SCCA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SCCA2. You can calculate the concentration of Mouse SCCA2 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
18.75 pg/mL
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Detection Range
31.25~2000pg/mL
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Target Name
SCCA2
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UNIProt ID
Q9JLI8
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Mouse SCCA2(Squamous Cell Carcinoma Antigen 2) EIA Kit
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Type
Sandwich
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Description
Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
SERPINB4
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Short name
Mouse SCCA2(Squamous Carcinoma Antigen 2) EIA Kit
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Technique
antigen, Mouse, EIA, antigenes, eia, mouses
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Host
mouse
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Species
Mouse, Mouses
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Alternative name
Mouse SCCA2(Squamous cellular Carcinoma protein 2) EIA reagent
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Alternative technique
antigenes, murine, kits, elisas
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
carcinoma, cell
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Gene info
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Identity
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Gene
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Long gene name
serpin family B member 4
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Synonyms gene
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Synonyms gene name
- squamous cell carcinoma antigen 2
- serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 4
- serpin peptidase inhibitor, clade B (ovalbumin), member 4
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Synonyms
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GenBank acession
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Locus
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Discovery year
1995-09-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serpin peptidase inhibitors
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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