SK-Hep-1 Cell Line
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Catalog numberEP-CL-0212
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationSK-Hep-1
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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Age52 years
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundThe SK-HEP-1 line has been identified as being of endothelial origin.
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Biosafety1
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MediumMEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicYes
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EffectsYes, in nude mice forms large cell carcinoma consistent with hepatoma.
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionNA
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ApplicationsTransfection host.
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolTMOD4, DKK1, NECTIN1
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Short nameSK-Hep-1 Line
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SpeciesHomo sapiens, Human
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Alternative nameSK-Hep-1 cellular Line
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TissueLiver/ascites
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Gene info
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Identity
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Gene
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Long gene nametropomodulin 4
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Synonyms gene name
- tropomodulin 4 (muscle)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-02-16
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Entrez gene record
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Pubmed identfication
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Classification
- Tropomodulins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namedickkopf WNT signaling pathway inhibitor 1
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Synonyms gene name
- dickkopf (Xenopus laevis) homolog 1
- dickkopf 1 homolog (Xenopus laevis)
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Synonyms
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Locus
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Discovery year2000-09-01
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenectin cell adhesion molecule 1
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Synonyms gene
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Synonyms gene name
- poliovirus receptor-related 1 (herpesvirus entry mediator C)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-11-15
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Entrez gene record
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Pubmed identfication
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Classification
- C2-set domain containing
- V-set domain containing
- Nectins and nectin-like molecules
- CD molecules
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VEGA ID