Hep 3B2.1-7 Cell Line

• Catalog number
  EP-CL-0102
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  Hep 3B2.1-7
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  8 years juvenile
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  This line contains an integrated hepatitis B virus genome.
• Biosafety
  2
• Medium
  MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, forms tumors in nude mice.
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  Alpha Fetoprotein(Alpha-Fetoprotein); Hepatitis B Surface Antigen(Hbsag); Albumin; Alpha2 Macroglobulin(Alpha-2-Macroglobulin); Alpha1 Antitrypsin(Alpha-1-Antitrypsin); Transferrin;, Alpha1 Antichymotrypsin(Alpha-1-Antichymotrypsin); Haptoglobin; Ceruloplasmin; Plasminogen; Complement(C3, C4); C3 Activator; Fibrinogen; Alpha1 Acid Glycoprotein(Alpha-1 Acid Glycoprotein);, Alpha2 Hs Glycoprotein(Alpha-2-Hs-Glycoprotein); Beta Lipoprotein(Beta-Lipoprotein); Retinol Binding Protein(Retinol-
• Applications
  Transfection host.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Hep   3B2   1-7   Line  
• Gene symbol
  MIR7-1, TRE-CTC1-7, TRV-CAC1-7, TRL-CAG1-7, PIRC34, PIRC33, PIRC32, TRK-TTT7-1, TRR-CCT7-1, TRI-AAT7-1
• Short name
  Hep 3B2 1-7 Line
• Species
  Homo sapiens, Human
• Alternative name
  Hep 3B2.1-7 cellular Line
• Tissue
  Liver

Gene info

• Identity
  HGNC:31638
• Gene
  MIR7-1
• Long gene name
  microRNA 7-1
• Synonyms gene
  • MIRN7-1
• Synonyms
  • hsa-mir-7-1
• Locus
  9q21.32
• Discovery year
  2004-04-23
• Entrez gene record
  407043
• RefSeq identity
  • NR_029605
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:34832
• Gene
  TRE-CTC1-7
• Long gene name
  tRNA-Glu (anticodon CTC) 1-7
• Synonyms gene
  • TRNAE19
• Synonyms gene name
  • transfer RNA glutamic acid 19 (anticodon CUC)
  • transfer RNA-Glu (CTC) 1-7
• Synonyms
  • tRNA-Glu-CTC-1-7
• GenBank acession
  • HG983759
• Locus
  6q22.31
• Discovery year
  2008-08-29
• Entrez gene record
  100189269
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:54846
• Gene
  TRV-CAC1-7
• Long gene name
  tRNA-Val (anticodon CAC) 1-7
• Synonyms
  • tRNA-Val-CAC-1-7
• Locus
  1q21.2
• Discovery year
  2020-02-13
• Entrez gene record
  100189228
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34854
• Gene
  TRL-CAG1-7
• Long gene name
  tRNA-Leu (anticodon CAG) 1-7
• Synonyms gene
  • TRNAL26
• Synonyms gene name
  • transfer RNA leucine 26 (anticodon CAG)
  • transfer RNA-Leu (CAG) 1-7
• Synonyms
  • tRNA-Leu-CAG-1-7
• GenBank acession
  • HG983895
• Locus
  6p22.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189288
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:37538
• Gene
  PIRC34
• Long gene name
  piwi-interacting RNA cluster 34
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313890
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37537
• Gene
  PIRC33
• Long gene name
  piwi-interacting RNA cluster 33
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313854
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37536
• Gene
  PIRC32
• Long gene name
  piwi-interacting RNA cluster 32
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313811
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:34900
• Gene
  TRK-TTT7-1
• Long gene name
  tRNA-Lys (anticodon TTT) 7-1
• Synonyms gene
  • TRNAK29
• Synonyms gene name
  • transfer RNA lysine 29 (anticodon UUU)
  • transfer RNA-Lys (TTT) 7-1
• Synonyms
  • tRNA-Lys-TTT-7-1
• GenBank acession
  • HG983939
• Locus
  6p22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189332
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:35013
• Gene
  TRR-CCT7-1
• Long gene name
  tRNA-Arg (anticodon CCT) 7-1
• Synonyms gene
  • TRNAR30P
• Synonyms gene name
  • transfer RNA arginine 30 (anticodon CCU) pseudogene
  • transfer RNA-Arg (CCT) 7-1
• Synonyms
  • tRNA-Arg-CCT-7-1
• GenBank acession
  • HG983612
• Locus
  1q21.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189443
• RefSeq identity
  • NG_008499
• Classification
  • Cytoplasmic transfer RNA pseudogenes

Gene info

• Identity
  HGNC:34710
• Gene
  TRI-AAT7-1
• Long gene name
  tRNA-Ile (anticodon AAT) 7-1
• Synonyms gene
  • TRNAI8
• Synonyms gene name
  • transfer RNA isoleucine 8 (anticodon AAU)
  • transfer RNA-Ile (AAT) 7-1
• Synonyms
  • tRNA-Ile-AAT-7-1
• GenBank acession
  • HG983853
• Locus
  6p22.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189401
• Classification
  • Cytoplasmic transfer RNAs

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