RKO-E6 Cell Line

• Catalog number
  EP-CL-0426
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  RKO-E6
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  NA
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  The cells contain a stably integrated human papilloma virus(HPV) E6 oncogene under control of the cytomegalovirus(CMV) promoter. The HPV E6 oncogene causes a decrease in normal p53 levels and functions.The RKO-E6 cell line lacks appreciable functional p53.Disruption of normal p53 function in human colon carcinoma RKO cells with the human papillomavirus E6 oncoprotein results in reduced repair of u.v.-induced DNA damage and also loss of induced repair following cellular u.v.-irradiation.RKO cells contain wild-type p53 but lack endogenous human thyroid receptor nuclear receptor(h-TRbeta1). The level of p53 protein is higher in RKO cells than in RKO-E6 cells.The RKO-E6 cell line can be used together with its parental cell line, RKO to investigate the effects of p53 loss on cellular parameters such as p53 mediated transcription and apoptosis.
• Biosafety
  2
• Medium
  MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  RKO-E6   Line  
• Gene symbol
  UBE3A
• Short name
  RKO-E6 Line
• Species
  Homo sapiens, Human
• Alternative name
  RKO-E6 cellular Line
• Tissue
  Colon

Gene info

• Identity
  HGNC:12496
• Gene
  UBE3A
• Long gene name
  ubiquitin protein ligase E3A
• Synonyms gene
  • EPVE6AP
  • HPVE6A
• Synonyms gene name
  • human papilloma virus E6-associated protein
• Synonyms
  • AS
  • ANCR
  • E6-AP
  • FLJ26981
• Synonyms name
  • Angelman syndrome
• GenBank acession
  • AF002224
• Locus
  15q11.2
• Discovery year
  1993-10-21
• Entrez gene record
  7337
• Pubmed identfication
  • 8221889
• RefSeq identity
  • NM_000462
• Classification
  • HECT domain containing
• VEGA ID
  OTTHUMG00000171548
• Locus Specific Databases
  • NGRL, Manchester LOVD
  • LRG_15

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