-
Abbreviation
RKO
-
Leadtime
3-5 days
-
Shipping condition
Dry ice
-
Storage
Liquid nitrogen -196°C
-
Age
NA
-
Morphology
Epithelial
-
Growth_properties
Adherent
-
Doubling_time
NA
-
Background
RKO cells contain wild-type p53 but lack endogenous human thyroid receptor nuclear receptor(h-TRbeta1). The level of p53 protein is higher in RKO cells than in RKO-E6 cells. The RKO cell line is the parental cell line(isogenic) of RKO-E6 and RKO-A545-1.
-
Biosafety
1
-
Medium
MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
-
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
-
Ratio
1:2-1:4
-
Renewal
Every 2 to 3 days
-
Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
-
Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
-
Tumorigenic
Yes
-
Effects
Yes, in nude mice. Yes, in soft agar.
-
Receptor_expression
Urokinase receptor(u-PAR)
-
Antigen_expression
NA
-
Gene_expression
NA
-
Applications
It can be used as the control cell line for investigating the effects of p53 and gadd45 on cellular parameters.
-
Duration
NA
-
-
Description
For cells, cell lines and tissues in culture till half confluency.