Recombinant Rat Fibroblast growth factor 2/Fibroblast Growth Factor Basic Protein
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Catalog numberPKSR030423-10µg
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PricePlease ask
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Size10µg
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SynonymFibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB
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ActivityMeasured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.The ED50 for this effect is less than 5 ng/ml.
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SequenceAla11-Ser154
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Fusion tagNA
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AccessionP13109
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ExpressedHostE.coli
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ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
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Purity>95% as determined by reducing SDS-PAGE.
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Stability and StorageLyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
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Mol Mass16.2 kDa
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AP Mol Mass16 kDa
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FormulationLyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl,pH7.4.
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BackgroundFGF-basic is a members of the Fibroblast Growth Factors (FGFs) family. The family constitutes a large family of proteins involved in many aspects of development including cell proliferation, growth, and differentiation. They act on several cell types to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects, and tissue repair. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4.
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DescriptionBasic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolFGF13, FGFBP2, API5
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Short nameRecombinant Fibroblast growth factor 2/Fibroblast Growth Factor Protein
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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HostRat
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SpeciesRat, Rats
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Alternative nameRec. Rat Fibroblast growth factor 2/Fibroblast Growth Factor Basic Protein
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namefibroblast growth factor 13
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Synonyms gene
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Synonyms gene name
- long intergenic non-protein coding RNA 889
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-12-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fibroblast growth factor family
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefibroblast growth factor binding protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameapoptosis inhibitor 5
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-10-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data