Recombinant Rat ESAM / Endothelial Cell Adhesion Molecule Protein (Fc tag)

• Catalog number
  PKSR030296-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  ESAM
• Activity
  NA
• Sequence
  Met 1-Ala 251
• Fusion tag
  C-Fc
• Accession
  Q6AYD4
• ExpressedHost
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 85 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  51.2 kDa
• AP Mol Mass
  55-60 KDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  Endothelial cell-selective adhesion molecule (ESAM) is a member of JAM family of immunoglobulin superfamily and consists of one V-type and one C2-type immunoglobulin domain, as well as a hydrophobic signal sequence, a single transmembrane region, and a cytoplasmic domain. It is specifically expressed at endothelial tight junctions and on activated platelets. ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall, possibly by influencing endothelial cell contacts. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been identified as an intracellular binding partner of ESAM. Previous studies have indicated that ESAM regulates angiogenesis in the primary tumor growth and endothelial permeability. It suggest that ESAM has a redundant functional role in physiological angiogenesis but serves a unique and essential role in pathological angiogenic processes such as tumor growth.
• Technical data
• Description
  cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Recombinant ESAM / Endothelial Adhesion Molecule Protein (Fc tag). For cells, cell lines and tissues in culture till half confluency. Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ESAM   Endothelial   Adhesion   Molecule   Protein   tag  
• Gene symbol
  ESAM, ESAM-AS1
• Short name
  Recombinant ESAM / Endothelial Adhesion Molecule Protein (Fc tag)
• Technique
  Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  Rat
• Label
  Fc Tag
• Species
  Rat, Rats
• Alternative name
  Rec. Rat endothelial cell adhesion molecule / Endothelial cellular Adhesion Molecule Protein (fragment c detection labelled)
• Alternative technique
  rec
• Alternative to gene target
  endothelial cell adhesion molecule, ESAM and IDBG-75276 and ENSG00000149564 and 90952, protein binding, Cell surfaces, Esam and IDBG-150411 and ENSMUSG00000001946 and 69524, BT.54007 and IDBG-642621 and ENSBTAG00000001004 and 616926
• Tissue
  cell

Gene info

• Identity
  HGNC:17474
• Gene
  ESAM
• Long gene name
  endothelial cell adhesion molecule
• Synonyms
  • W117m
• GenBank acession
  • AK075396
• Locus
  11q24.2
• Discovery year
  2003-11-26
• Entrez gene record
  90952
• Pubmed identfication
  • 11279107
  • 11906820
• RefSeq identity
  • NM_138961
• Classification
  • IgCAM CXADR-related subfamily
  • V-set domain containing
• VEGA ID
  OTTHUMG00000151986

Gene info

• Identity
  HGNC:55558
• Gene
  ESAM-AS1
• Long gene name
  ESAM antisense RNA 1
• Locus
  11q24.2
• Discovery year
  2021-04-07
• Entrez gene record
  101929340
• RefSeq identity
  • NR_120577
• Classification
  • Antisense RNAs

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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