Recombinant Rat CD5 Protein (His tag) 100µg
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Catalog number
PKSR030198-100µg
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Price
Please ask
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Size
100µg
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Synonym
CD5
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Activity
Immobilized rat CD5-His at 10 μg/ml (100 μl/well) can bind biotinylated human GST-LCK (Cat:10043-H09B), The EC50 of biotinylated human GST-LCK is 0.9-2.1 μg/ml.
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Sequence
Met1-Pro368
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Fusion tag
C-His
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Accession
P51882
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ExpressedHost
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
> 95 % as determined by SDS-PAGE
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Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
38.9 kDa
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AP Mol Mass
46-49 KDa
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Formulation
Lyophilized from sterile PBS, pH 7.4
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Background
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.
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Conjugation
histidine
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CD5
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Short name
Recombinant CD5 Protein (His tag) 100µg
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
Rat
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Label
His
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Species
Rat, Rats
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Alternative name
Rec. Rat CD5 molecule Protein (histidine detection labelled) 100µg
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Alternative technique
rec
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Alternative to gene target
CD5 molecule, LEU1 and T1, CD5 and IDBG-49730 and ENSG00000110448 and 921, protein binding, Cell surfaces, Cd5 and IDBG-144910 and ENSMUSG00000024669 and 12507, CD5 and IDBG-643103 and ENSBTAG00000013730 and 280745
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Gene info
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Identity
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Gene
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Long gene name
CD5 molecule
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Synonyms gene
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Scavenger receptor cysteine rich domain containing
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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