Recombinant Mouse SPG21 / ACP33 Protein (His & GST tag)
-
Catalog number
PKSM040487-20µg
-
Price
Please ask
-
Size
20µg
-
-
Synonym
ACP33;BM-019;C78576;D9Wsu18e;GL010;MAST
-
Activity
NA
-
Sequence
Met 1-Pro 308
-
Fusion tag
N-His & GST
-
Accession
Q9CQC8-1
-
Expressed Host
Baculovirus-Insect Cells
-
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
-
Purity
> 90 % as determined by SDS-PAGE
-
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
-
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Mol Mass
62.8kDa
-
AP Mol Mass
52 kDa
-
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 3mM DTT, 10% glycerol
-
Background
Spastic paraplegia 21 (SPG21), also known as acid Cluster Protein 33 (ACP33) and Mast syndrome protein, is a member of the AB hydrolase superfamily. Human SPG21 is a 308 amino acid residue protein widely expressed in all tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. SPG21 binds to the hydrophobic C-terminal amino acids of CD4 which are involved in repression of T cell activation via the noncatalytic alpha/beta hydrolase fold domain. SPG21 thus is proposed to play a role as a negative regulatory factor in CD4-dependent T-cell activation of CD4. Defects in SPG21 are the cause of spastic paraplegia autosomal recessive type 21, also known as Mast syndrome, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. SPG21 is also associated with dementia and other central nervous system abnormalities.
-
-
Conjugation
histidine
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
SPG21, MGST3
-
Short name
Recombinant Mouse SPG21 / ACP33 Protein (His & GST tag)
-
Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
-
Host
mouse
-
Label
His
-
Species
Mouse, Mouses
-
Alternative name
Rec. Mouse spastic paraplegia 21 (autosomal recessive, Mast syndrome) / ACP33 Protein (histidine & GST detection labelled)
-
Alternative technique
rec, murine
-
Alternative to gene target
spastic paraplegia 21 (autosomal recessive, Mast syndrome), SPG21 and IDBG-16930 and ENSG00000090487 and 51324, CD4 receptor binding, nuclei, Spg21 and IDBG-177905 and ENSMUSG00000032388 and 27965, SPG21 and IDBG-645311 and ENSBTAG00000011540 and 404069
-
Gene info
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
