Recombinant Mouse CD64 / FCGR1 Protein (His & AVI tag) 100µg
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Catalog numberPKSM040878-100µg
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Price:
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Size100µg
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ActivityMeasured by its binding ability in a functional ELISA. Immobilized mouse CD64-AVI at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.07-0.15 μg/ml.
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SynonymAI323638;AV092959;CD64;FcgammaRI;IGGHAFC
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SequenceMet 1-Pro 297
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Fusion tagC-His & AVI
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AccessionNP_034316.1
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Expressed HostHEK293 Cells
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ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity> 97 % as determined by SDS-PAGE
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Endotoxin< 1.0 EU per μg of the protein as determined by the LAL method
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Stability and StorageSamples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass34.3 kDa
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AP Mol Mass45-50 kDa
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FormulationLyophilized from sterile PBS, pH 7.4
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BackgroundHigh affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.
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Conjugationhistidine
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolFCGR1A
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Short nameRecombinant Mouse CD64 / FCGR1 Protein (His & AVI tag) 100µg
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TechniqueRecombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Hostmouse
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LabelHis
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SpeciesMouse, Mouses
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Alternative nameRec. Mouse CD64 / FCGR1 Protein (histidine & AVI detection labelled) 100µg
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Alternative techniquerec, murine
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Gene info
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Identity
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Gene
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Long gene nameFc gamma receptor Ia
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Synonyms gene name
- Fc fragment of IgG, high affinity Ia, receptor for (CD64)
- Fc fragment of IgG, high affinity Ia, receptor (CD64)
- Fc fragment of IgG receptor Ia
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1992-12-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data