Recombinant Mouse beta-Catenin / CTNNB1 Protein (His & GST tag)

#

• Catalog number
  PKSM040590-10µg
• Price:

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• Size
  10µg

# #

• Synonym
  Bfc;Catnb;Mesc
• Activity
  NA
• Sequence
  Met1-Leu781
• Fusion tag
  N-His & GST
• Accession
  Q02248
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 90 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  113 kDa
• AP Mol Mass
  115 kDa
• Formulation
  Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
• Background
  beta-Catenin, also known as CTNNB1, is a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain, which is specialized for protein-protein binding. It is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. CTNNB1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, beta-Catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Defects in beta-Catenin can cause colorectal cancer, pilomatrixoma (PTR), medulloblastoma, and ovarian cancer. CTNNB1 is a key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
• Technical data
• Conjugation
  histidine
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

#

• Gene target
  beta-Catenin   CTNNB1   Protein   His   GST   tag  
• Gene symbol
  MGST3, CTNNB1
• Short name
  Recombinant Mouse beta-Catenin / CTNNB1 Protein (His & GST tag)
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  mouse
• Label
  His
• Species
  Mouse, Mouses
• Alternative name
  Rec. Mouse b-Catenin / catenin (cadherin-associated protein), b 1, 88kDa Protein (histidine & GST detection labelled)
• Alternative technique
  rec, murine
• Alternative to gene target
  catenin (cadherin-associated protein), beta 1, 88kDa, armadillo and CTNNB and MRD19, CTNNB1 and IDBG-27347 and ENSG00000168036 and 1499, repressing transcription factor binding, nuclei, Ctnnb1 and IDBG-205243 and ENSMUSG00000006932 and 12387, CTNNB1 and IDBG-644470 and ENSBTAG00000016420 and 539003

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

Gene info

• Identity
  HGNC:2514
• Gene
  CTNNB1
• Long gene name
  catenin beta 1
• Synonyms gene
  • CTNNB
• Synonyms gene name
  • catenin (cadherin-associated protein), beta 1 (88kD)
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin (cadherin-associated protein), beta 1
• Synonyms
  • beta-catenin
  • armadillo
• GenBank acession
  • X87838
• Locus
  3p22.1
• Discovery year
  1993-07-13
• Entrez gene record
  1499
• Pubmed identfication
  • 7829088
• RefSeq identity
  • NM_001098210
• Classification
  • Beta catenins
  • Wnt enhanceosome complex
  • Armadillo repeat containing
• VEGA ID
  OTTHUMG00000131393
• Locus Specific Databases
  • LRG_1108

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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