Recombinant Human beta-Catenin / CTNNB1 Protein (His & GST tag)
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Catalog numberPKSH031171-50µg
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Price:
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Size50µg
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Synonymarmadillo;CTNNB;MRD19
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ActivityNA
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SequenceMet 1-Leu 781
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Fusion tagN-His & GST
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AccessionP35222-1
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Expressed HostBaculovirus-Insect Cells
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ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity>85 % as determined by SDS-PAGE
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Endotoxin<1.0 EU per µg as determined by the LAL method
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Stability and StorageSamples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass113 kDa
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AP Mol Mass116 kDa
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FormulationLyophilized from sterile 50mM Tris, 100mM NaCl, 2mM GSH, 10% gly, pH 8.0
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Backgroundbeta-Catenin, also known as CTNNB1, is a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain, which is specialized for protein-protein binding. It is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. CTNNB1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, beta-Catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Defects in beta-Catenin can cause colorectal cancer, pilomatrixoma (PTR), medulloblastoma, and ovarian cancer. CTNNB1 is a key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
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Conjugationhistidine
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolMGST3, CTNNB1
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Short nameRecombinant beta-Catenin / CTNNB1 Protein (His & GST tag)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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LabelHis
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SpeciesHuman, Humans
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Alternative nameRec. H. sapiens b-Catenin / catenin (cadherin-associated protein), b 1, 88kDa Protein (histidine & GST detection labelled)
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Alternative techniquerec
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Alternative to gene targetcatenin (cadherin-associated protein), beta 1, 88kDa, armadillo and CTNNB and MRD19, CTNNB1 and IDBG-27347 and ENSG00000168036 and 1499, repressing transcription factor binding, nuclei, Ctnnb1 and IDBG-205243 and ENSMUSG00000006932 and 12387, CTNNB1 and IDBG-644470 and ENSBTAG00000016420 and 539003
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Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecatenin beta 1
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Synonyms gene
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Synonyms gene name
- catenin (cadherin-associated protein), beta 1 (88kD)
- catenin (cadherin-associated protein), beta 1, 88kDa
- catenin (cadherin-associated protein), beta 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-07-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Beta catenins
- Wnt enhanceosome complex
- Armadillo repeat containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data