Recombinant Mouse Angiotensinogen / SerpinA8 / AGT Protein (His tag)
-
Catalog number
PKSM040726-50µg
-
Price
Please ask
-
Size
50µg
-
-
Synonym
AI265500;AngI;AngII;Aogen;Serpina8
-
Activity
NA
-
Sequence
Met 1-Val 477
-
Fusion tag
C-His
-
Accession
P11859
-
Expressed Host
HEK293 Cells
-
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
-
Purity
> 96 % as determined by SDS-PAGE
-
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
-
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Mol Mass
50.8 kDa
-
AP Mol Mass
58 kDa
-
Formulation
Lyophilized from sterile PBS, pH 7.4
-
Background
Angiotensinogen, also known as AGT and SerpinA8, is a member of the serpin family. It is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. Angiotensinogen can be schematically considered to consist of a combination of an angiotensin I (Ang I) function, located at the N-terminal end, and the presence of a serpin (serine protease inhibitor) structure at the opposite end. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD). Several serpins (antithrombin, maspin, pigment epithelial-derived factor, and kallistatin) have been recently shown to exert an antiangiogenic activity, suggesting a common mechanism of endothelial cell proliferation and migration. Angiotensinogen/AGT and its renin-cleaved product, des(Ang I)AGT, are also angiogenesis inhibitors, both in vitro and in vivo at concentrations within the range of those observed in plasma. The Angiotensinogen products, that is angiotensin II and possibly angiotensin II-related products, have been found to act locally in modulating adipose tissue growth in an autocrine/paracrine manner. The transient or chronic overexpression of angiotensinogen in adipose tissue favors lipogenesis in adipocytes and leads to a 'vicious' circle whereby adipose tissue development is further increased.
-
-
Conjugation
histidine
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
AGT, SLC7A13, AGXT, TRT-AGT3-1, TRT-AGT2-1, TRT-AGT2-2, TRT-AGT1-3, TRT-AGT7-1, TRT-AGT1-1, TRT-AGT1-2
-
Short name
Recombinant Mouse Angiotensinogen / SerpinA8 / AGT Protein (His tag)
-
Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
-
Host
mouse
-
Label
His
-
Species
Mouse, Mouses
-
Alternative name
Rec. Mouse Angiotensinogen / SerpinA8 / angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Protein (histidine detection labelled)
-
Alternative technique
rec, murine
-
Alternative to gene target
angiotensinogen (serpin peptidase inhibitor, clade A, member 8), ANHU and SERPINA8, AGT and IDBG-107363 and ENSG00000135744 and 183, type 2 angiotensin receptor binding, Extracellular, Agt and IDBG-200169 and ENSMUSG00000031980 and 11606, AGT and IDBG-632058 and ENSBTAG00000012393 and 527114
-
Gene info
-
Identity
-
Gene
-
Long gene name
angiotensinogen
-
Synonyms gene
-
Synonyms gene name
- serpin peptidase inhibitor, clade A, member 8
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1989-06-30
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Neuropeptides
- Serpin peptidase inhibitors
-
VEGA ID
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
-
Identity
-
Gene
-
Long gene name
tRNA-Thr (AGT) 7-1
-
Synonyms gene
-
Synonyms gene name
- tRNA threonine (AGU) 3
- transfer RNA threonine 3 (anticodon AGU)
- transfer RNA-Thr (AGT) 7-1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year
1993-05-24
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Low confidence cytoplasmic transfer RNAs
Gene info
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
