Recombinant Human SMYD3 / ZMYND1 Protein (His & FLAG tag)

• Catalog number
  PKSH031188-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  bA74P14.1;KMT3E;ZMYND1;ZNFN3A1
• Activity
  NA
• Sequence
  Met 1-Ser 428
• Fusion tag
  C-His & N-FLAG
• Accession
  NP_001161212.1
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞70 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  51.5 kDa
• AP Mol Mass
  51.5 kDa
• Formulation
  Lyophilized from sterile 25mM Tris-HCl, 100mM NaCl, 20% glycerol, 3mM DTT, pH 8.0
• Background
  SET and MYND domain-containing protein 3, also known as Zinc finger MYND domain-containing protein 1, SMYD3, and ZMYND, is a member of the histone-lysine methyltransferase family. SMYD3 contains one MYND-type zinc finger and one SET domain. SMYD3 is a histone H3 lysine-4-specific methyltransferase. It is expressed in skeletal muscles and testis. It is overexpressed in a majority of colorectal carcinoma (CRC) and hepatocellular carcinoma (HCC). SMYD3 plays an important role in transcriptional regulation in human carcinogenesis. It activates the transcription of a set of downstream genes. Of these downstream genes, there are several oncogenes and genes associated with cell adhesion (including those of N-Myc, CrkL, Wnt10b, L-selectin, CD31 and galectin-4), which have been shown to have effects on cell viability, adhesion, migration and metastasis. Increased SMYD3 expression is essential for the proliferation of breast cancer cells. SMYD3 may be a promising new target of therapeutic intervention for the treatment of cancers or other pathological processes associated with cell adhesion and migration.
• Technical data
• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Flag, histidine
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  SMYD3   ZMYND1   Protein   His   tag  
• Gene symbol
  SMYD3, SMYD3-AS1, SMYD3-IT1
• Short name
  Recombinant SMYD3 / ZMYND1 Protein (His & tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens SMYD3 / ZMYND1 Protein (histidine & FLAG detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:15513
• Gene
  SMYD3
• Long gene name
  SET and MYND domain containing 3
• Synonyms gene
  • ZNFN3A1
  • ZMYND1
• Synonyms gene name
  • zinc finger, MYND domain containing 1
• Synonyms
  • KMT3E
• GenBank acession
  • AK023594
• Locus
  1q44
• Discovery year
  2001-04-06
• Entrez gene record
  64754
• RefSeq identity
  • NM_022743
• Classification
  • SET domain containing
  • Lysine methyltransferases
  • Zinc fingers MYND-type
• VEGA ID
  OTTHUMG00000040508

Gene info

• Identity
  HGNC:40708
• Gene
  SMYD3-AS1
• Long gene name
  SMYD3 antisense RNA 1
• Locus
  1q44
• Discovery year
  2020-03-27
• Entrez gene record
  101928089
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:41406
• Gene
  SMYD3-IT1
• Long gene name
  SMYD3 intronic transcript 1
• Synonyms gene name
  • SMYD3 intronic transcript 1 (non-protein coding)
• Locus
  1q44
• Discovery year
  2011-08-23
• Classification
  • Intronic transcripts
• VEGA ID
  OTTHUMG00000040509

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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