Recombinant Human CD40 Ligand/CD40L/TNFSF5 Protein
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Catalog number
PKSH032214-10µg
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Price
Please ask
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Size
10µg
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Synonym
CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP
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Activity
NA
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Sequence
Met113-Leu261
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Fusion tag
NA
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Accession
P29965
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Expressed Host
Human cells
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Shipping
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
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Purity
>95% as determined by reducing SDS-PAGE.
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Endotoxin
<1.0 EU per µg as determined by LAL test.
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Stability and Storage
Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
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Mol Mass
16.2 kDa
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AP Mol Mass
16-18 kDa
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Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.
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Background
CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Description
FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CD40LG, CD40
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Short name
Recombinant CD40 Ligand/CD40L/TNFSF5 Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Rec. H. sapiens CD40 molecule, tumor necrosis factor receptor superfamily member 5 Ligand/CD40L/TNFSF5 Protein
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Alternative technique
rec
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Alternative to gene target
CD40 molecule, TNF receptor superfamily member 5, Bp50 and CDW40 and p50 and TNFRSF5, CD40 and IDBG-78904 and ENSG00000101017 and 958, ubiquitin protein ligase binding, Extracellular, Cd40 and IDBG-212533 and ENSMUSG00000017652 and 21939, BT.42498 and IDBG-643036 and ENSBTAG00000020736 and 286849
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Gene info
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Identity
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Gene
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Long gene name
CD40 ligand
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Tumor necrosis factor superfamily
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene name
CD40 molecule
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor receptor superfamily, member 5
- CD40 molecule, TNF receptor superfamily member 5
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Synonyms
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GenBank acession
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Locus
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Discovery year
1993-08-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Tumor necrosis factor receptor superfamily
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VEGA ID
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Locus Specific Databases
Product images
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