Recombinant Human CD16a / FCGR3A Protein (176 Phe, His tag)
-
Catalog number
PKSH030287-50µg
-
Price
Please ask
-
Size
50µg
-
-
Synonym
CD16;CD16A;Fc gamma RIIIa;FCG3;FCGR3;FCGRIII;FCR-10;FCRIII;FCRIIIA;IGFR3;IMD20
-
Activity
Measured by its binding ability in a functional ELISA. Immobilized human CD16a-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.90-2.30 μg/ml.
-
Sequence
Met 1-Gln 208
-
Fusion tag
C-His
-
Accession
P08637-1
-
Expressed Host
HEK293 Cells
-
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
-
Purity
>97 % as determined by SDS-PAGE
-
Endotoxin
<1.0 EU per µg as determined by the LAL method
-
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Mol Mass
23.3 kDa
-
AP Mol Mass
45-50 kDa
-
Formulation
Lyophilized from sterile PBS, pH 7.4
-
Background
The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
-
-
Conjugation
histidine
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
FCGR3A
-
Short name
Recombinant CD16a / FCGR3A Protein (176 Phe, His tag)
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
-
Label
His
-
Species
Human, Humans
-
Alternative name
Rec. H. sapiens CD16a / fragment c fragment on Immunoglobulin G, low affinity IIIa, receptor (CD16a) Protein (176 L-phenylalanine, histidine detection labelled)
-
Alternative technique
rec
-
Alternative to gene target
Fc fragment of IgG, low affinity IIIa, receptor (CD16a), CD16 and CD16A and FCG3 and FCGR3 and FCGRIII and FCR-10 and FCRIII and FCRIIIA and IGFR3 and IMD20, FCGR3A and IDBG-104292 and ENSG00000203747 and 2214, IgG binding, Cell surfaces
-
Gene info
-
Identity
-
Gene
-
Long gene name
Fc gamma receptor IIIa
-
Synonyms gene
-
Synonyms gene name
- Fc fragment of IgG, low affinity IIIa, receptor for (CD16)
- Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
- Fc fragment of IgG receptor IIIa
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year
1988-11-30
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Immunoglobulin like domain containing
- CD molecules
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products