Recombinant Human CD16a / FCGR3A Protein (176 Phe, His & AVI tag), Biotinylated

• Catalog number
  PKSH030284-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  CD16;CD16A;Fc gamma RIIIa;FCG3;FCGR3;FCGRIII;FCR-10;FCRIII;FCRIIIA;IGFR3;IMD20
• Activity
  Measured by its ability to bind recombinant human IgG1 in a functional ELISA.2. Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD16a. The EC50 of Human CD16a is 2.4 - 5.7 μg/ml.3. Labeling ratio of biotin to protein: 0.7-1.
• Sequence
  Met 1-Gln 208
• Fusion tag
  C-His & AVI
• Accession
  P08637-1
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞90 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  25.1 kDa
• AP Mol Mass
  48 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CD16a   FCGR3A   Protein   176   Phe   His   AVI   tag   Biotinylated  
• Gene symbol
  FCGR3A
• Short name
  Recombinant CD16a / FCGR3A Protein (176 Phe, His & AVI tag), Biotinylated
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens CD16a / fragment c fragment on Immunoglobulin G, low affinity IIIa, receptor (CD16a) Protein (176 L-phenylalanine, histidine & AVI detection labelled), Biotinylated
• Alternative technique
  rec
• Alternative to gene target
  Fc fragment of IgG, low affinity IIIa, receptor (CD16a), CD16 and CD16A and FCG3 and FCGR3 and FCGRIII and FCR-10 and FCRIII and FCRIIIA and IGFR3 and IMD20, FCGR3A and IDBG-104292 and ENSG00000203747 and 2214, IgG binding, Cell surfaces

Gene info

• Identity
  HGNC:3619
• Gene
  FCGR3A
• Long gene name
  Fc gamma receptor IIIa
• Synonyms gene
  • FCGR3
  • FCG3
• Synonyms gene name
  • Fc fragment of IgG, low affinity IIIa, receptor for (CD16)
  • Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
  • Fc fragment of IgG receptor IIIa
• Synonyms
  • CD16
  • CD16a
  • FcgammaRIIIa
  • FcGRIIIA
• GenBank acession
  • BC036723
• Locus
  1q23.3
• Discovery year
  1988-11-30
• Entrez gene record
  2214
• Pubmed identfication
  • 2139735
  • 11835346
  • 19309690
• RefSeq identity
  • NM_000569
• Classification
  • Immunoglobulin like domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000034466
• Locus Specific Databases
  • FCGR3Abase: Mutation registry for Natural killer cell deficiency
  • LRG_60

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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