Recombinant Human Carbonic Anhydrase II / CA2 Protein (His tag)

• Catalog number
  PKSH031582-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  CA-II;CAC;CAII;Car2;HEL-76;HEL-S-282
• Activity
  Measured by its esterase activity. The specific activity is >150 pmoles/min/μg.
• Sequence
  Met 1-Lys 260
• Fusion tag
  C-His
• Accession
  NP_000058.1
• Expressed Host
  E. coli
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞96 % as determined by SDS-PAGE
• Endotoxin
  Please contact us for more information.
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  30 kDa
• AP Mol Mass
  30 kDa
• Formulation
  Lyophilized from sterile 20mM Tris 0.5M NaCl, pH 8.0
• Background
  The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Carbonic   Anhydrase   CA2   Protein   His   tag  
• Gene symbol
  CA2
• Short name
  Recombinant Carbonic Anhydrase II / CA2 Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Carbonic Anhydrase II / CA2 Protein (histidine detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:1373
• Gene
  CA2
• Long gene name
  carbonic anhydrase 2
• Synonyms gene name
  • carbonic anhydrase II
• Synonyms
  • Car2
  • CA-II
  • CAII
• GenBank acession
  • J03037
• Locus
  8q21.2
• Discovery year
  2001-06-22
• Entrez gene record
  760
• Pubmed identfication
  • 3107918
• RefSeq identity
  • NM_000067
• Classification
  • Carbonic anhydrases
• VEGA ID
  OTTHUMG00000164944

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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