Rat PDIA3(Protein Disulfide Isomerase A3) EIA Kit

• Catalog number
  E-EL-R0802-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Rat
• Target Synonym
  NA
• Test principle
  This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat PDIA3. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PDIA3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PDIA3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PDIA3. You can calculate the concentration of Rat PDIA3 in the samples by comparing the OD of the samples to the standard curve.
• Sensitivity
  1.88ng/mL
• Detection Range
  3.13~200ng/mL
• Target Name
  PDIA3
• UNIProt ID
  P11598
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Rat PDIA3(Protein Disulfide Isomerase A3) EIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Description
  This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  PDIA3   Protein   Disulfide   Isomerase   Kit  
• Gene symbol
  PDIA3
• Short name
  PDIA3(Protein Disulfide Isomerase A3) EIA Kit
• Technique
  EIA, eia
• Host
  Rat
• Species
  Rat, Rats
• Alternative name
  Rat protein disulfide isomerase family A, member 3(Protein Disulfide Isomerase A3) EIA reagent
• Alternative technique
  kits, elisas
• Alternative to gene target
  protein disulfide isomerase family A, member 3, ER60 and ERp57 and ERp60 and ERp61 and GRP57 and GRP58 and HEL-S-269 and HEL-S-93n and HsT17083 and P58 and PI-PLC, PDIA3 and IDBG-9160 and ENSG00000167004 and 2923, poly(A) RNA binding, nuclei, Pdia3 and IDBG-202293 and ENSMUSG00000027248 and 14827, PDIA3 and IDBG-633244 and ENSBTAG00000017196 and 281803

Gene info

• Identity
  HGNC:4606
• Gene
  PDIA3
• Long gene name
  protein disulfide isomerase family A member 3
• Synonyms gene
  • GRP58
• Synonyms gene name
  • glucose regulated protein, 58kDa
  • protein disulfide isomerase-associated 3
  • protein disulfide isomerase family A, member 3
• Synonyms
  • P58
  • ERp61
  • ERp57
  • ERp60
  • GRP57
  • PI-PLC
  • HsT17083
• Locus
  15q15.3
• Discovery year
  1997-06-09
• Entrez gene record
  2923
• Pubmed identfication
  • 8974399
• RefSeq identity
  • NM_005313
• Classification
  • Protein disulfide isomerases
• VEGA ID
  OTTHUMG00000044444

MeSH Data

• Name
  Immunoenzyme Techniques
• Concept
  Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
• Tree numbers
  • E05.478.566.350
  • E05.478.583.400
  • E05.601.470.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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