NCTC clone 929 [L cell, L-929, derivative of Strain L] Cell Line

Abbreviation

NCTC clone 929 [L cell, L-929, derivative of Strain L]
Leadtime

3-5 days
Shipping condition

Dry ice
Storage

Liquid nitrogen -196°C
Age

100 days
Morphology

Fibroblast
Growth_properties

Adherent
Doubling_time

NA
Background

This cell line has been tested and found negative for ectromelia virus(mousepox).
Biosafety

1
Medium

MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
Subculturing

Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio

1:2-1:4
Renewal

Every 2 to 3 days
Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture_conditions

Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic

Yes, in immunosuppressed mice.
Effects

Yes, in immunosuppressed mice.
Receptor_expression

NA
Antigen_expression

NA
Gene_expression

NA
Applications

Toxicity testing. Transfection host
Duration

NA
Technical data
Description

For cells, cell lines and tissues in culture till half confluency.

Gene target

NCTC 929 L-929 derivative Strain Line
Gene symbol

CPT1A, POM121L2, L3MBTL2, CLDN10, RPL32P1, FABP1, COX7A2, NFIA, MAS1L, ANP32E
Short name

NCTC clone 929 [L , L-929, derivative of Strain L] Line
Species

Mus musculus, Mouse
Alternative name

NCTC clonality 929 [L cellular, L-929, derivative on Strain L] cellular Line
Clone name

929
Tissue

Subcutaneous connective tissue, areolar and adipose
Virus

strain

Identity

HGNC:13973
Gene

POM121L2
Long gene name

POM121 transmembrane nucleoporin like 2
Synonyms gene name
- POM121 membrane glycoprotein-like 2 (rat)
- POM121 membrane glycoprotein-like 2
- POM121 transmembrane nucleoporin-like 2
Synonyms
- POM121-L
GenBank acession
- AL021808
Locus

6p22.1
Discovery year

2001-07-20
Entrez gene record

94026
RefSeq identity
- NM_033482
VEGA ID

OTTHUMG00000014475

Identity

HGNC:18594
Gene

L3MBTL2
Long gene name

L3MBTL histone methyl-lysine binding protein 2
Synonyms gene name
- l(3)mbt-like 2 (Drosophila)
- L3MBTL2, polycomb repressive complex 1 subunit
Synonyms
GenBank acession
- AJ305226
Locus

22q13.2
Discovery year

2002-04-29
Entrez gene record

83746
Pubmed identfication
- 11682070
- 21596310
RefSeq identity
- NM_031488
Classification
- MBT domain containing
VEGA ID

OTTHUMG00000150942

Identity

HGNC:16673
Gene

ANP32E
Long gene name

acidic nuclear phosphoprotein 32 family member E
Synonyms gene name
- acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
Synonyms
- LANPL
- MGC5350
- LANP-L
GenBank acession
- AK092672
Locus

1q21.2
Discovery year

2003-04-15
Entrez gene record

81611
Pubmed identfication
- 12438741
RefSeq identity
- NM_030920
Classification
- ANP32 acidic nuclear phosphoproteins
VEGA ID

OTTHUMG00000012547