Human FcγR Ⅱ/CD32(Receptor Ⅱ for the Fc Fragment of IgG) EIA Kit
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Catalog number
E-EL-H1743-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Human
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Target Synonym
NA
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human FcγR Ⅱ/CD32. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FcγR Ⅱ/CD32 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FcγR Ⅱ/CD32, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FcγR Ⅱ/CD32. You can calculate the concentration of Human FcγR Ⅱ/CD32 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
28.13pg/mL
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Detection Range
46.88~3000pg/mL
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Target Name
FcγR Ⅱ/CD32
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UNIProt ID
P31994
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Human FcγR Ⅱ/CD32(Receptor Ⅱ for the Fc Fragment of IgG) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Gene symbol
FCGR2B, FCGR2A
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Short name
FcγR Ⅱ/CD32(Receptor Ⅱ for the Fc of IgG) EIA Kit
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Technique
EIA, IgG, FC, eia, IgGs
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Isotype
IgG, Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common. IgG
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Species
Humans
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Alternative name
H. sapiens FcγR Ⅱ/CD32(Receptor Ⅱ to measure the fragment c Fragment on Immunoglobulin G) EIA reagent
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Alternative technique
kits, elisas, immunoglobulins
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
Fc gamma receptor IIb
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Synonyms gene
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Synonyms gene name
- Fc fragment of IgG, low affinity IIb, receptor for (CD32)
- Fc fragment of IgG, low affinity IIb, receptor (CD32)
- Fc fragment of IgG receptor IIb
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Synonyms
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GenBank acession
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Locus
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Discovery year
1991-08-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
Fc gamma receptor IIa
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Synonyms gene
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Synonyms gene name
- Fc fragment of IgG, low affinity IIa, receptor for (CD32)
- Fc fragment of IgG, low affinity IIa, receptor (CD32)
- Fc fragment of IgG receptor IIa
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1988-11-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin like domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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