CHO-K1 Cell Line
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Catalog numberEP-CL-0062
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationCHO-K1
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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AgeNA
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundThe cells require Proline in the medium for growth.The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957. The cells require Proline in the medium for growth.
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Biosafety1
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MediumHam's F-12K (PM150910)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicNA
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EffectsNA
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionNA
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ApplicationsTransfection host.
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolCOLEC11
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Short nameCHO-K1 Line
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Hostcho
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SpeciesCricetulus griseus, Hamster, Chinese
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Alternative nameCHO-K1 cellular Line
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TissueOvary
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Gene info
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Identity
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Gene
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Long gene namecollectin subfamily member 11
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-11-20
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Entrez gene record
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RefSeq identity
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Classification
- Collectins
- C-type lectin domain containing
- Complement system activation components
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VEGA ID
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Locus Specific Databases