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Long name
Rabbit Antibody against Rat Aif1, FITC
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Synonym name
Aif1 Rat Antibody
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Species reactivity
Rat
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Tested applications recommended dilutions
ELISA
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Conjugation
FITC
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Antibody isotype
IgG
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UniProt ID
P55009
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant rat Allograft inflammatory factor 1 protein (2-147AA)
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Immunogen species
Rat
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Alias
Allograft inflammatory factor 1,AIF-1,Ionized calcium-binding adapter molecule 1,MRF-1,Microglia response factor,Aif1,Iba1,Mrf1
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Background
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play an role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
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Clonality
Polyclonal antibody
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Shipping and storage
Aif1 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cusabio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cusabio FITC is highly sensitive to pH extremes.
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Additional conjugation
Anti-FITC Antibody
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Oryctolagus cuniculus, Rattus norvegicus
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French translation
anticorps
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Gene target
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Gene symbol
AIF1
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Short name
Anti-Aif1
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Technique
Antibody, Rabbit, anti, FITC, antibody to, antibodies against human proteins, antibodies for, Fluorescein
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Host
Rabbit, Rabbits
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Label
FITC
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Species
Rat, Rats
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Alternative name
Aif1 Antibody,FITC conjugated
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Alternative technique
antibodies, rabbit-anti, fluorescine
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Alternative to gene target
allograft inflammatory factor 1, AIF-1 and IBA1 and IRT-1 and IRT1, AIF1 and IDBG-78190 and ENSG00000204472 and 199, actin filament binding, nuclei, Aif1 and IDBG-177226 and ENSMUSG00000024397 and 11629, AIF1 and IDBG-634144 and ENSBTAG00000020554 and 280989
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