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Long name
Rabbit Antibody against Mouse Aif1, Biotin
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Synonym name
Aif1 Mouse Antibody
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Species reactivity
Mouse
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Tested applications recommended dilutions
ELISA
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Conjugation
Biotin
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Antibody isotype
IgG
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UniProt ID
O70200
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant mouse Allograft inflammatory factor 1 protein (2-147AA)
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Immunogen species
Mouse
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Alias
onized calcium-binding adapter molecule 1,Iba1,Aif1
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Background
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
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Clonality
Polyclonal antibody
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Shipping and storage
Aif1 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigen
Allograft inflammatory factor 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Additional conjugation
Biotinylated
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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French translation
anticorps
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Gene target
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Gene symbol
AIF1
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Short name
Anti-Aif1
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Technique
anti mouse, Antibody, Rabbit, Mouse, anti, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, mouses
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Host
Rabbit, Rabbits
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Label
Biotin
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Species
Mouse, Mouses
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Alternative name
Aif1 Antibody,Biotin conjugated
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Alternative technique
antibodies, rabbit-anti, murine
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Alternative to gene target
allograft inflammatory factor 1, AIF-1 and IBA1 and IRT-1 and IRT1, AIF1 and IDBG-78190 and ENSG00000204472 and 199, actin filament binding, nuclei, Aif1 and IDBG-177226 and ENSMUSG00000024397 and 11629, AIF1 and IDBG-634144 and ENSBTAG00000020554 and 280989
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