Rabbit Anti Human RNF40 Antibody-Biotin
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Catalog numberCSB-PA019890LD01HU_50ug
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Price:
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Size50ug
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Long nameRabbit Antibody against Human RNF40, Biotin
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Synonym nameRNF40 Human Antibody
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Species reactivityHuman
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Tested applications recommended dilutionsELISA
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ConjugationBiotin
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Antibody isotypeIgG
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UniProt IDO75150
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FormatLiquid, diluted in buffer
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Buffer compositionPreservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
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Purity>95%
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Purification methodProtein G purified
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Antibody categoryPolyclonal Antibodies
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Immunogen typeRecombinant Human E3 ubiquitin-protein ligase BRE1B protein (471-724AA)
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Immunogen speciesHuman
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AliasE3 ubiquitin-protein ligase BRE1B,BRE1-B,95 kDa retinoblastoma-associated protein,RBP95,RING finger protein 40,RNF40,BRE1B,KIAA0661
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BackgroundComponent of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.
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ClonalityPolyclonal antibody
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Shipping and storageRNF40 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigenE3 ubiquitin-protein ligase BRE1B
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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PropertiesIf you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional conjugationBiotinylated
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AboutRabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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French translationanticorps
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Gene target
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Gene symbolRNF40
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Short nameAnti-RNF40
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Techniqueanti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for
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HostRabbit, Rabbits
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LabelBiotin
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SpeciesHuman, Humans
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Alternative nameRNF40 Antibody,Biotin conjugated
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Alternative techniqueantibodies, rabbit-anti
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Gene info
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Identity
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Gene
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Long gene namering finger protein 40
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ring finger proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data