-
Long name
Rabbit Antibody against Human RIPK2, HRP
-
Synonym name
RIPK2 Human Antibody
-
Species reactivity
Human
-
Tested applications recommended dilutions
ELISA
-
Conjugation
HRP
-
Antibody isotype
IgG
-
UniProt ID
O43353
-
Format
Liquid, diluted in buffer
-
Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
-
Purity
>95%
-
Purification method
Protein G purified
-
Antibody category
Polyclonal Antibodies
-
Immunogen type
Recombinant human Receptor-interacting serine/threo nine-protein kinase 2 protein (1-540AA)
-
Immunogen species
Human
-
Alias
CARD-containing interleukin-1 beta-converting enzyme-associated kinase RIP-like-interacting CLARP kinase Receptor-interacting protein 2 Tyrosine-protein kinase RIPK2 EC=2.7.10.2 RIPK2 CARDIAK, RICK, RIP2 UNQ277/PRO314/PRO34092
-
Background
Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
-
Clonality
Polyclonal antibody
-
Shipping and storage
RIPK2 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
-
Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional conjugation
HRP Conjugated
-
About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
French translation
anticorps
-
Gene target
-
Gene symbol
RIPK2-DT, RIPK2
-
Short name
Anti-RIPK2
-
Technique
anti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for
-
Host
Rabbit, Rabbits
-
Label
HRP
-
Species
Human, Humans
-
Alternative name
RIPK2 Antibody,HRP conjugated
-
Alternative technique
antibodies, rabbit-anti
-
Alternative to gene target
receptor-interacting serine-threonine kinase 2, CARD3 and CARDIAK and CCK and GIG30 and RICK and RIP2, RIPK2 and IDBG-27941 and ENSG00000104312 and 8767, transferase activity, Cytoplasm, Ripk2 and IDBG-130745 and ENSMUSG00000041135 and 192656, RIPK2 and IDBG-645358 and ENSBTAG00000015271 and 534407
-