-
Long name
Rabbit Antibody against Human EPHA2, HRP
-
Synonym name
EPHA2 Human Antibody
-
Species reactivity
Human
-
Tested applications recommended dilutions
ELISA
-
Conjugation
HRP
-
Antibody isotype
IgG
-
UniProt ID
P29317
-
Format
Liquid, diluted in buffer
-
Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
-
Purity
>95%
-
Purification method
Protein G purified
-
Antibody category
Polyclonal Antibodies
-
Immunogen type
Recombinant Human Ephrin type-A receptor 2 protein (627-976AA)
-
Immunogen species
Human
-
Alias
Ephrin type-A receptor 2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, EPHA2, ECK
-
Background
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
-
Clonality
Polyclonal antibody
-
Shipping and storage
EPHA2 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
-
Short name of the antigen
Ephrin type-A receptor 2
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
-
Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional conjugation
HRP Conjugated
-
About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
French translation
anticorps
-
Gene target
-
Gene symbol
EPHA2, EPHA2-AS1
-
Short name
Anti-EPHA2
-
Technique
anti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for
-
Host
Rabbit, Rabbits
-
Label
HRP
-
Species
Human, Humans
-
Alternative name
EPHA2 Antibody,HRP conjugated
-
Alternative technique
antibodies, rabbit-anti
-
Alternative to gene target
EPH receptor A2, ARCC2 and CTPA and CTPP1 and CTRCT6 and ECK, EPHA2 and IDBG-91407 and ENSG00000142627 and 1969, transferase activity, Cell surfaces, Epha2 and IDBG-202005 and ENSMUSG00000006445 and 13836, EPHA2 and IDBG-645870 and ENSBTAG00000000815 and 512798
-
