Rabbit Anti Human APAF1 Antibody-FITC

• Catalog number
  CSB-PA001885LC01HU_100ug
• Price
  Please ask
• Size
  100ug

• Long name
  Rabbit Antibody against Human APAF1, FITC
• Synonym name
  APAF1 Human Antibody
• Species reactivity
  Human
• Tested applications recommended dilutions
  ELISA
• Conjugation
  FITC
• Antibody isotype
  IgG
• UniProt ID
  O14727
• Format
  Liquid, diluted in buffer
• Buffer composition
  Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity
  >95%
• Purification method
  Protein G purified
• Antibody category
  Polyclonal Antibodies
• Immunogen type
  Recombinant human Apoptotic protease-activating factor 1 protein (104-415AA)
• Immunogen species
  Human
• Alias
  Apoptotic protease-activating factor 1,APAF-1,APAF1,KIAA0413
• Background
  Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
• Clonality
  Polyclonal antibody
• Shipping and storage
  APAF1 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Properties
  If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cusabio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cusabio FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional conjugation
  Anti-FITC Antibody
• About
  Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  APAF1  
• Gene symbol
  APAF1
• Short name
  Anti-APAF1
• Technique
  anti human, Antibody, Rabbit, anti, FITC, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, Fluorescein
• Host
  Rabbit, Rabbits
• Label
  FITC
• Species
  Human, Humans
• Alternative name
  APAF1 Antibody,FITC conjugated
• Alternative technique
  antibodies, rabbit-anti, fluorescine
• Alternative to gene target
  apoptotic peptidase activating factor 1, APAF-1 and CED4, APAF1 and IDBG-52364 and ENSG00000120868 and 317, ADP binding, nuclei, Apaf1 and IDBG-185032 and ENSMUSG00000019979 and 11783, APAF1 and IDBG-636787 and ENSBTAG00000021661 and 537782

Gene info

• Identity
  HGNC:576
• Gene
  APAF1
• Long gene name
  apoptotic peptidase activating factor 1
• Synonyms gene name
  • apoptotic protease activating factor
  • apoptotic peptidase activating factor
• Synonyms
  • CED4
  • APAF-1
• GenBank acession
  • AF013263
• Locus
  12q23.1
• Discovery year
  1998-01-20
• Entrez gene record
  317
• Pubmed identfication
  • 9267021
  • 10702682
• RefSeq identity
  • NM_181861.1
• Classification
  • Apoptosome
  • WD repeat domain containing
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000170214

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products