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Description
Recombinant Human cytomegalovirus Enhanced green fluorescent protein(egfp) expressed in Yeast
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Specifications
Uniprot ID: C5MKY7; Host: Human cytomegalovirus (HHV-5) (Human herpesvirus 5); Expression system: Yeast; Expression region: 1-239aa; Sequence information: Full Length; Tag: N-terminal 10xHis-tagged and C-terminal Myc-tagged; Purity: Greater than 90% as determined by SDS-PAGE.
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Additional_information
MW: 30.9 kDa; Buffer composition: Tris-based buffer with 50% glycerol.
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Storage_and_shipping
Shipped on ice packs (+4°C). The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Repeated freezing and thawing should be avoided. Store working aliquots at 4°C for up to one week.
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Notes
For research use only.
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Conjugate
The Green Fluorescent Protein (eGFP) of cytomegalovirus Enhanced protein ( ) can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.
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Additional description
Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
