Recombinant Caspase Activated DNase (CAD)

Source

Prokaryotic expression
Predicted Molecular Mass KD

31.9kDa
Accurate Molecular Mass KD

NA
Organism species

Homo sapiens (Human)
Item Name

Caspase Activated DNase
Alternative Names

DFF-B; DFFB; CPAN; DFF40; DFF2; DNA Fragmentation Factor 40kDa Beta Polypeptide; Caspase-activated deoxyribonuclease; Caspase-activated nuclease
Research Area

Apoptosis;
Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Traits

Freeze-dried powder
Isoelectric Point

8.4
Purity

≥ 95%
Protein length

Arg87~Lys323
Tag

two N-terminal Tags, His-tag and T7-tag
Expression System

Prokaryotic expression
Application

Positive Control; Immunogen; SDS-PAGE; WB.
Manual link
Description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Additional source

Recombinants or rec. proteins
Group

recombinants

Gene target

Caspase Activated DNase CAD
Gene symbol

CALD1, CAD, B4GALNT2, ACOD1, CDH12P2, DFFB
Short name

Recombinant Caspase Activated DNase (CAD)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Cloud Clone Corp advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Host

E.coli
Species

Homo sapiens (Human)
Alternative name

Rec. caspase Activated DNase (CAD)
Alternative technique

rec

Identity

HGNC:1424
Gene

CAD
Long gene name

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Synonyms
- GATD4
GenBank acession
- D78586
Locus

2p23.3
Discovery year

2001-06-22
Entrez gene record

790
Pubmed identfication
- 8619816
- 2565865
Classification
- Glutamine amidotransferase class 1 domain containing
VEGA ID

OTTHUMG00000097070

Identity

HGNC:24136
Gene

B4GALNT2
Long gene name

beta-1,4-N-acetyl-galactosaminyltransferase 2
Synonyms gene
- GALGT2
Synonyms gene name
- UDP-GalNAc:Neu5Acalpha2-3Galbeta-R beta1,4-N-acetylgalactosaminyltransferase
Synonyms
- Sda
- Cad
GenBank acession
- AJ517770
Locus

17q21.32
Discovery year

2004-02-20
Entrez gene record

124872
Pubmed identfication
- 8782649
- 12678917
RefSeq identity
- NM_153446
Classification
- Beta 4-glycosyltransferases
VEGA ID

OTTHUMG00000161307

Identity

HGNC:1752
Gene

CDH12P2
Long gene name

cadherin 12 pseudogene 2
Synonyms gene
- CDH12P
Synonyms gene name
- cadherin 12 (N-cadherin 2) pseudogene
- cadherin 12 (N-cadherin 2) pseudogene 2
Synonyms
- C41-CAD
GenBank acession
- AC004999
Locus

5q13.2
Discovery year

1995-11-30
Entrez gene record

1011
Pubmed identfication
- 7731968
- 10369862
VEGA ID

OTTHUMG00000162392

Identity

HGNC:2773
Gene

DFFB
Long gene name

DNA fragmentation factor subunit beta
Synonyms gene name
- DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
Synonyms
- CAD
- CPAN
- DFF-40
- DFF40
Synonyms name
- caspase-activated DNase
Locus

1p36.32
Discovery year

1997-10-27
Entrez gene record

1677
Pubmed identfication
- 9108473
- 9560346
RefSeq identity
- NM_001282669
VEGA ID

OTTHUMG00000003525

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data