Perforin 1 (PRF1) Polyclonal Antibody (Mouse), FITC
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Catalog number
PAB317Mu01-20ul-FITC
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Price
Please ask
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Size
20ul
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Description
A Rabbit polyclonal antibody against Mouse Perforin 1 (PRF1). This antibody is labeled with FITC.
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Specifications
Host: Rabbit; Species Reactivity: Mouse; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
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Additional_information
Sequence of the immunogen: PRF1 (Val40~Lys355); Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
PRF1
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Short name
Perforin 1 (PRF1) Polyclonal Antibody (Mouse), FITC
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Technique
Polyclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
mouse
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Label
FITC
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Species
Mouse, Mouses
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Alternative name
Perforin 1 (perforin 1 (pore forming protein)) polyclonal (antibody to-) (Mouse), fluorecein
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Alternative technique
polyclonals, antibodies, murine, fluorescine
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Alternative to gene target
perforin 1 (pore forming protein), FLH2 and HPLH2 and P1 and PFN1 and PFP, PRF1 and IDBG-77451 and ENSG00000180644 and 5551, wide pore channel activity, Extracellular, Prf1 and IDBG-155739 and ENSMUSG00000037202 and 18646, BT.21975 and IDBG-629772 and ENSBTAG00000001008 and 369025
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Gene info
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Identity
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Gene
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Long gene name
perforin 1
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Synonyms gene name
- perforin 1 (pore forming protein)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1989-02-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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