Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody (Rat), FITC

• Catalog number
  PAB663Ra01-10ml-FITC
• Price
  Please ask
• Size
  10ml

• Description
  A Rabbit polyclonal antibody against Rat Lymphocyte Activation Gene 3 (LAG3). This antibody is labeled with FITC.
• Specifications
  Host: Rabbit; Species Reactivity: Rat; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
• Additional_information
  Sequence of the immunogen: LAG3 (Asn209~Leu451); Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Storage_and_shipping
  Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
• Notes
  Research Use Only.
• Properties
  If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Additional description
  cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• French translation
  anticorps

• Gene target
  Lymphocyte   Activation   Gene   LAG3  
• Gene symbol
  LAG3
• Short name
  Lymphocyte Activation 3 (LAG3) Polyclonal Antibody ( ), FITC
• Technique
  activation, Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rat
• Label
  FITC
• Species
  Rat, Rats
• Alternative name
  Lymphocyte Activation Gene 3 (lymphocyte-activation gene 3) polyclonal (antibody to-) (Rat), fluorecein
• Alternative technique
  polyclonals, antibodies, fluorescine
• Alternative to gene target
  lymphocyte-activation gene 3, CD223, LAG3 and IDBG-14994 and ENSG00000089692 and 3902, MHC class II protein binding, Cell surfaces, Lag3 and IDBG-188462 and ENSMUSG00000030124 and 16768, BT.18959 and IDBG-640276 and ENSBTAG00000034295 and 515155
• Tissue
  lymphocyte

Gene info

• Identity
  HGNC:6476
• Gene
  LAG3
• Long gene name
  lymphocyte activating 3
• Synonyms gene name
  • lymphocyte-activation gene 3
• Synonyms
  • CD223
• Locus
  12p13.31
• Discovery year
  1991-06-03
• Entrez gene record
  3902
• RefSeq identity
  • NM_002286
• Classification
  • Immunoglobulin like domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000169197

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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