Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) Polyclonal Antibody (Human, Mouse, Pig), FITC
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Catalog numberPAB091Hu01-1ml-FITC
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Price:
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Size1ml
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DescriptionA Rabbit polyclonal antibody against Human, Mouse, Pig Alkaline Phosphatase, Liver/Bone/Kidney (ALPL). This antibody is labeled with FITC.
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SpecificationsHost: Rabbit; Species Reactivity: Human, Mouse, Pig; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
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Additional_informationSequence of the immunogen: ALPL (Leu18~Arg335); Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
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Storage_and_shippingUpon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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NotesResearch Use Only.
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PropertiesIf you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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AboutPigs and the smaller guinea pigs are frequent used as models for humans. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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GroupPolyclonals and antibodies
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French translationanticorps
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Gene target
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Gene symbolALPL
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Short nameAlkaline Phosphatase, Liver/Bone/Kidney (ALPL) Polyclonal Antibody ( , Mouse, ), FITC
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TechniquePolyclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Hostmouse
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LabelAlkaline Phosphatase
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SpeciesPig, Humans, Mouses, Pigs and Guinea pigs
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Alternative nameAlkaline Phosphatase, Liver/Bone/Kidney (alkaline phosphatase, liver/bone/kidney) polyclonal (antibody to-) (H. sapiens, Mouse, swine), fluorecein
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Alternative techniquepolyclonals, antibodies, murine, fluorescine
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Alternative to gene targetalkaline phosphatase, liver/bone/kidney, AP-TNAP and APTNAP and HOPS and TNAP and TNSALP, ALPL and IDBG-93194 and ENSG00000162551 and 249, metal ion binding, Extracellular, Alpl and IDBG-199139 and ENSMUSG00000028766 and 11647, ALPL and IDBG-645614 and ENSBTAG00000008951 and 280994
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Tissuebone, kidney, liver
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Gene info
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Identity
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Gene
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Long gene namealkaline phosphatase, biomineralization associated
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Synonyms gene
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Synonyms gene name
- alkaline phosphatase, liver/bone/kidney
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Alkaline phosphatases
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data