Total Phosphatidic Acid Assay test kit (Fluorimetry)

• Catalog number
  MET-5019
• Price
  Please ask
• Size
  100 assays

• We send this reagant on
  Dry Ice
• The temperatureerature for storage is
  Store the entire test kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorimetry Probe is light sensitive and should be maintained in amber tubes.
• Technical file
  more kit-Fluorimetry.pdf
• SDS or safty data sheet
  details (MET-5019) GHS.pdf
• Type of reagent
  Metabolism Research
• Related techniques
  Metabolism Research, Lipoprotein Metabolism, Phospholipid Assays
• Use of the reagent
  Our Total Phosphatidic Acid Assay test kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the test kit’s Fluorimetry Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.
• Cellular use
  Detects total phosphatidic acid content (PA and LPA) in cell lysate samples
• Protocol information
  Detection sensitivity of approximately 5 uM phosphatidic acid
• Reagent properties
  Phosphatidic Acid standard curve included
• Conjugation and form
  host specific
• Species compatible to
  human cells and cell lines and other cells
• Use of this test kit or product
  Molecular biology
• Antigen
  the sequence of the protein
• Buffer
  The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,4
• Molecular weight
  kiloDalton
• This reagent is purified
  0.98
• Test
  Cellular biology labs supplies other types of Assays as 1.

• Gene target
  Phosphatidic   Acid   test   kit   Fluorimetry  
• Short name
  Phosphatidic Acid Assay test kit (Fluorimetry)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Total Phosphatidic Acid test test reagent (Fluorimetry)
• Alternative technique
  arrays, tests, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  total

Similar products