Total Phosphatidic Acid Assay Kit (Fluorometric)

Shipping Temp

Dry Ice
Storage Temp

Store the entire kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.
Category Product Type

Kit
URL link to datasheet
URL link to SDS

more (MET-5019) GHS.pdf
UNSPSC

41116100
Product Line

Metabolism Research
Short Description

Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.
Product feature 1

Detects total phosphatidic acid content (PA and LPA) in cell lysate samples
Product feature 2

Detection sensitivity of approximately 5 uM phosphatidic acid
Product feature 3

Phosphatidic Acid standard curve included
Test

Cell Biolabs supplies other types of Assays as 1.

Gene target

Phosphatidic Acid Kit Fluorometric
Short name

Phosphatidic Acid Assay Kit (Fluorometric)
Technique

Assay, assays
Host

Assay
Alternative name

Total Phosphatidic Acid test reagent (fluorescence)
Alternative technique

arrays, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Tissue

total