RAPAd® miRNA Adenoviral Expression System
#
-
Catalog numberVPK-253
-
Price:
-
Size1 test kit
-
-
We send this reagant onBlue Ice
-
The temperatureerature for storage is-20ºC
-
SDS or safty data sheetmore (VPK-250, 251, 252, 253, 254, 255, 256) GHS.pdf
-
Type of reagentViral Expression
-
Related techniquesmicroRNA Analysis, miRNA Expression and Reporter Vectors, miRNA Viral Expression Vectors
-
Use of the reagentMaking an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.
-
Cellular useEngineered to produce 100- to 10,000-fold fewer wild type plaques than most other expression systems
-
Protocol informationGenerate high-titer virus in about 2-3 weeks compared to 2-3 months with other systems
-
Reagent propertiesCloning capacity of shuttle vector = 5.2 kb
-
Living modified organisme in this reagent or LMONot applicable
-
Species compatible toHumans
-
Use of this test kit or productGenetics lab
-
Antigena specific protein sequence
-
BufferThe concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,73
-
Molecular weightin kilo Danlto or kDa
-
This reagent is purified0.97
-
Gene target
-
Short nameRAPAd® miRNA Adenoviral Expression System
-
TechniquemiRNA, mirna, microRNAs
-
Alternative nameRAPAd® RNA silencing Adenoviral Expression System
-
Alternative techniquemicrornas
-
MeSH Data
-
Name
-
ConceptScope note: Process of growing viruses in live animals, plants, or cultured cells.
-
Tree numbers
- E01.370.225.875.970
- E05.200.875.970
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data