RAPAd® CMV Adenoviral Expression System

Shipping Temp

Blue Ice
Storage Temp

-20ºC
Category Product Type

Kit
URL link to datasheet
URL link to SDS

more (VPK-250, 251, 252, 253, 254, 255, 256) GHS.pdf
UNSPSC

41105908
Product Line

Viral Expression
Short Description

Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.
Product feature 1

Engineered to produce 100- to 10,000-fold fewer wild type plaques than most other expression systems
Product feature 2

Generate high-titer virus in about 2-3 weeks compared to 2-3 months with other systems
Product feature 3

Cloning capacity of shuttle vector = 6.9 kb

Name

Virus Cultivation
Concept

Scope note: Process of growing viruses in live animals, plants, or cultured cells.
Tree numbers
- E01.370.225.875.970
- E05.200.875.970
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data