OxiSelect™ Cellular Antioxidant Assay test kit (Green Fluorescence)

• Catalog number
  STA-349
• Price
  Please ask
• Size
  192 assays

• We send this reagant on
  Room temperature
• The temperatureerature for storage is
  Upon receipt, store the DCFH-DA Probe (1000X) and Quercetin at -20ºC. Avoid multiple freeze/thaw cycles. Store the store the remaining components at 4ºC.
• Technical file
  more kit.pdf
• SDS or safty data sheet
  details (STA-349) GHS.pdf
• Type of reagent
  Oxidative Stress/Damage
• Related techniques
  Oxidative Stress/ Damage, Antioxidant Assays, Cellular Antioxidant Assay
• Use of the reagent
  The OxiSelect™ Cellular Antioxidant Assay test kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells.  Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested.  After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator.  The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF.  The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.
• Cellular use
  Determine the efficacy of an exogenous antioxidant compound within a cellular environment
• Protocol information
  Each test kit provides sufficient reagents to perform up to 192 assays, including standard curve and unknown samples
• Species compatible to
  Humans
• Use of this test kit or product
  Genetics lab
• Antigen
  a specific protein sequence
• Buffer
  The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,7
• Molecular weight
  in kilo Danlto or kDa
• This reagent is purified
  0.97
• Test
  Cellular biology labs supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.

• Gene target
  OxiSelect™   Cellular   Antioxidant   test   kit  
• Short name
  OxiSelect™ Cellular Antioxidant Assay test kit ( )
• Technique
  cellular, Assay, assays
• Host
  Assay
• Label
  green
• Alternative name
  OxiSelect™ Cellular Antioxidant test test reagent (Green Fluorescence)
• Alternative technique
  arrays, tests, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cellular

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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