OxiSelect Cellular Antioxidant Assay Kit (Green Fluorescence)

• Catalog number
  STA-349
• Price
  Please ask
• Size
  192 reactions

• Type of the product
  Set of reagents
• Product description
  The OxiSelect Cellular Antioxidant Assay Set of reagents is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells.  Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested.  After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator.  The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF.  The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.
• Shipping conditions
  The product OxiSelect Cellular Antioxidant Assay Kit (Green Fluorescence)can be shipped at ambient temperature without any risk for the quality and activity of the reagents.
• Storage conditions
  After the delivery of this product, our specialists from Gentaur/Genprice recommend you to prepare aliquots to minimize the risk of repeated cycles of freezing and thawing. The standard suspension should be kept at minus twenty degrees Celsius but the rest of the reagents can be stored at four degrees Celsius.
• Tips
  This product is manufactured for research purposes only and it is not intended to be used in clinical diagnostic procedures.
• Test
  Cellular biology labs supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.

• Gene target
  OxiSelect   Cellular   Antioxidant   Kit  
• Short name
  OxiSelect Cellular Antioxidant Assay Kit ( )
• Technique
  cellular, Assay, assays
• Host
  Assay
• Label
  green
• Alternative name
  OxiSelect Cellular Antioxidant test reagent (Green Fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cellular

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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