Sterile filtered HEPES 1M solution (2283 g/L HEPES) in purified water

• Catalog number
  HOL06-6X100ML
• Price
  Please ask
• Size
  6 x 100 mL

• Availability
  Please contact us to check current availability
• Long description
  Sterile filtered HEPES 1M solution (2283 g/L HEPES) in purified water
• Product format
  Liquid
• Product category
  Biochemicals
• Product subcategory
  Buffers and Supplements
• Shipping method
  Ambient temperature
• Storage method
  Store at 2˚ to 8˚C.
• Other products
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
• Description
  The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous. This 1 has passed a sterilization (or sterilization) by filtration or inactivation that has eliminated (removed) or killed (deactivated) all forms of DNA, RNA and enzymes. Also life and other biological agents (such as viruses which some do not consider to be alive but are biological pathogens nonetheless), excluding prions which cannot be killed, including transmissible agents (such as fungi, bacteria, viruses, prions, spore forms, unicellular eukaryotic organisms such as Plasmodium, etc.) present in a specified reagent or on a surface, a volume of fluid, or in a compound such as biological culture medias filtered. Sterilization was achieved with one or more of the following heat, chemicals, irradiation, high pressure, and filtration. Sterilization is distinct from disinfection, sanitization, and pasteurization in that sterilization kills, deactivates, or eliminates all forms of life and other biological agents.

• Gene target
  filtered   2283   g/L   water  
• Short name
  HEPES
• Technique
  purified, sterile, purifications, sterile pure
• Alternative name
  Sterile filtered HEPES 1M solution (2283 g/L HEPES) in enriched water
• Alternative technique
  pure, sterilized

MeSH Data

• Name
  Filtration
• Concept
  Scope note: A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
• Tree numbers
  • E05.196.454
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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