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Target antigen
PKB alpha
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Clonality
Monoclonal antibody
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Clone
PKB-175
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Raised in
mice
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Type of the antibody
IgG1 monoclonal antibody
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Product form
freeze-dried
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Reacts with species
Human, all kinds of animals
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Analyses
WB
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Immunogen
Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH.
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Product configuration
Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
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Purification
Ascites
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Solubilization
The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
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Storage condtions
Keep the PKB alpha Antibody ( PKB175)at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The PKB alpha Antibody ( PKB175) did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1(ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.
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Related articles
1. Staal, S. P.; Huebner, K.; Croce, C. M.; Parsa, N. Z.; Testa, J. R. : The AKT1 proto-oncogene maps to human chromosome 14, band q32. Genomics 2: 96-98, 1988. 2. Ackah, E.; Yu, J.; Zoellner, S.; Iwakiri, Y.; Skurk, C.; Shibata, R.; Ouchi, N.; Easton, R. M.; Galasso, G.; Birnbaum, M. J.; Walsh, K.; Sessa, W. C. : Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. J. Clin. Invest. 115: 2119-2127, 2005. 3. Chen, J.; Somanath, P. R.; Razorenova, O.; Chen, W. S.; Hay, N.; Bornstein, P.; Byzova, T. V. : Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo. Nature Med. 11: 1188-1196, 2005.
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Gene Name
AKT1
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Protein Name
RAC-alpha serine/threonine-protein kinase
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Gene Full Name
v-akt murine thymoma viral oncogene homolog 1
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Synonyms
AKT|PKB|RAC|CWS6|PRKBA|PKB-ALPHA|RAC-ALPHA|ONCOGENE AKT1|PROTEIN KINASE B-ALPHA|RAC SERINE/THREONINE PROTEIN KINASE|Protein kinase B|Proto-oncogene c-Akt|RAC-PK-alpha
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Uniprot ID
Human(P31749), Mouse(P31750), Rat(P47196)
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Entrez GeneID
Human(207), Mouse(11651), Rat(24185)
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Description
The PKB alpha Antibody ( PKB175) is a α- or alpha protein sometimes glycoprotein present in blood.
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
