-
ELISA s application
For quantitative measurement of human TGF alpha in cell culture supernatant, serum, plasma heparin, EDTA and human milk.
-
Gene tested
Human TGF alpha
-
ELISA standard curve
Concentration(pg/ml) + 0 + 15.6 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.102 + 0.199 + 0.291 + 0.443 + 0.711 + 1.156 + 1.676 + 2.315
-
ELISA kit detection limits
15.6pg/ml to 1000pg/ml
-
ELISA s sensitivity
< 1pg/ml
-
Target of this ELISA
Natural and recombinant human TGF alpha
-
Cross reaction
There is cross-reactivates with TGF beta 2, TGF beta 3, TGF beta 5
-
ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);118;225;516;126;238;509 ELISA standard deviations 5.43;12.83;16.5;8.95;14.28;27 CV(%);4.6;5.7;3.2;7.1;6;5.3
-
ELISA kit description
GENTAUR's human TGF alpha ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for TGF alpha has been precoated onto 96-well plates. Standards (E.coli,V40-A89) and your samples are measured with a biotin conjugated polyclonal from goat specific for TGF alpha will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human TGF alpha in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
-
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
-
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human TGF alpha antibodies;1 ST0000-10;Lyophilized recombinant human TGF alpha standard;10ng/tubex2 AR1107;Biotinylated anti- human TGF alpha antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
-
Anti coagulation
heparin or EDTA
-
How the elisa works
Sample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml human TGF alpha standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum, plasma(heparin, EDTA) or milk to the ELISA plate. Keep in mind that each human TGF alpha standard solution and each sample be measured in duplicate.
-
Minutes of TMB incubation
25-30
-
More gene info
Transforming growth factor alpha(TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to human chromosome 2 close to the breakpoint of the t(2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.
-
Antigen
E.coli,V40-A89
-
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
-
Uniprot
Protransforming growth factor alpha
-
Gene name
transforming growth factor, alpha
-
Alternative
EGF like TGF;_x000D_ETGF;_x000D_OTTHUMP00000203300;_x000D__x000D__x000D_OTTHUMP00000203301;_x000D_OTTHUMP00000203345;_x000D_OTTHUMP00000203352;_x000D_Protransforming growth factor alpha;_x000D_RATTGFAA;_x000D_TFGA;_x000D_TGF A;_x000D_TGF type 1;_x000D_TGFA;_x000D_Transforming growth factor alpha;_x000D_Transforming growth factor alpha precursor;_x000D_Wa1;_x000D_Waved 1
-
Gen protein identity
P01135
-
Entrez nr
7039
-
Description
The TGF alpha PicoKine ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
-
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
-
Gene target
-
Gene symbol
TGFA
-
Short name
TGF alpha PicoKine ELISA Kit
-
Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
-
Host
antigen assay ELISA kit 1 Kit (96 Wells)
-
Species
Human, Humans
-
Alternative name
H. sapiens TGF a PicoKine Enzyme-linked immunosorbent assay reagent
-
Alternative technique
elisas, kits
-
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
-
