-
ELISA s application
For quantitative measurement of human Marapsin in cell culture supernatant and serum.
-
Gene tested
human Marapsin
-
ELISA standard curve
Concentration(pg/ml) + 0 + 125 + 250 + 500 + 1000 + 2000 + 4000 + 8000 O.D. + 0.029 + 0.060 + 0.124 + 0.195 + 0.432 + 0.834 + 1.369 + 1.897
-
ELISA kit detection limits
125pg/ml to 8000pg/ml
-
ELISA s sensitivity
< 10pg/ml
-
Target of this ELISA
Natural and recombinant human Marapsin
-
Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
-
ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);736;2858;4299;823;2914;4567 ELISA standard deviations 41.21;197.2;279.43;60.08;227.3;383.63 CV(%);5.6;6.9;6.5;7.3;7.8;8.4
-
ELISA kit description
GENTAUR's human Marapsin ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for Marapsin was used as capure antibodies on the ELISA test plate. The internal standards (Expression system for standard: NSO, Immunogen sequence: M35-K290) and your samples are measured with a biotin conjugated polyclonal from goat specific for Marapsin will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human Marapsin in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
-
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
-
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human Marapsin antibodies;1 ST0000-10;Lyophilized recombinant human Marapsin standard;10ng/tube×2 AR1107;Biotinylated anti- human Marapsin antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
-
Anti coagulation
/
-
How the elisa works
Sample the amount of 100ul/well of the standard solution at 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human Marapsin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant or serum to the ELISA plate. Keep in mind that each human Marapsin standard solution and each sample be measured in duplicate.
-
Minutes of TMB incubation
15-20
-
More gene info
Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.
-
Antigen
NSO, M35-K290
-
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
-
Uniprot
Serine protease 27
-
Gene name
protease, serine 27
-
Alternative
CAPH2 ; Marapsin ; MPN ; Pancreasin ; Prss27 ; Prss 27 ; Protease serine 27 ; UNQ1884/PRO4327 ; Q9BQR3
-
Gen protein identity
Q9BQR3
-
Entrez nr
83886
-
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
-
Gene target
-
Gene symbol
PRSS27
-
Short name
Marapsin/Pancresin PicoKine ELISA Kit
-
Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
-
Host
antigen assay ELISA kit 1 Kit (96 Wells)
-
Species
Human, Humans
-
Alternative name
H. sapiens Marapsin/Pancresin PicoKine Enzyme-linked immunosorbent assay reagent
-
Alternative technique
elisas, kits
-
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
-